ABSTRACT
Objective:To explore the role of multimedia teaching based on post competency in practical teaching of pediatrics.Methods:A total of 64 pediatric interns received by our unit from January 2019 to October 2020 were studied and randomly divided into two groups. Traditional clinical teaching was used in the control group, and multimedia teaching based on post competency was used in the experimental group. The assessment results of pediatrics-related theoretical knowledge and practical skills, post competency and recognition of teaching were compared between the two groups before and after teaching. SPSS 26.0 was used for t-test and chi-square test. Results:After teaching, the examination results of the two groups of interns' pediatrics-related theoretical knowledge and practical operation skills were higher than those before teaching [(98.72±1.29) vs. (79.84±3.96), (97.94±2.17) vs. (70.22±4.17)], and the above results of the experimental group were higher than those of the control group [(98.72±1.29) vs. (90.22±3.46), (97.94±2.17) vs. (87.38±5.07)] ( P<0.05); the post competency scores of clinical diagnosis and treatment ability, clinical operation skills, self-learning and information technology, and medical humanistic quality of the two groups after teaching were higher than those before teaching, and the scores of the experimental group were higher than those of the control group ( P<0.05); the recognition rate of the teaching method in stimulating learning interest, improving learning effect, enhancing doctor-patient communication ability, rasing clinical thinking ability, and strenthening professional confidence in the experimental group was higher than that in the control group ( P<0.05). Conclusion:The application of multimedia teaching based on post competency in the practical teaching of pediatrics can not only improve the examination results and various aspects of post competency, but also be recognized by interns.
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of beta3-adrenergic receptor(beta3-AR) antagonist on myocardial uncoupling protein 2 (UCP2) expression and energy metabolism in chronic heart failure rats.</p><p><b>METHODS</b>Seven weight-matched normal adult rats (control group), 18 isoproterenol (ISO) induced heat failure (HR) rats (ISO group) and 21 ISO induced heart failure rats but received specific beta3-AR inhibitor SR59230A (ISO+ SR59230A group) for 6 weeks were included in this research. At the end of the study, echocardiography was performed, the ratio of left ventricular weight and body weight (LVW/BW) was calculated. The expression of beta3-AR ad UCP2 mRNA in myocardium were detected by reverse transcription-polymerase chain reaction (RT-PCR), the UCP2 protein in myocardium were detected by Western blot. The myocardial contents of creatine phosphate (PCr) and adenosine triphosphate (ATP) were measured by high performance liquid chromatography (HPLC).</p><p><b>RESULTS</b>Compared with control group, the cardiac function was significantly reduced and myocardial beta3-AR mRNA significantly increased, UCP2 mRNA and protein were also significantly increased in ISO group, this change could be attenuated by the treatment with SR59230A, and the expression of myocardial UCP2 protein negatively correlated with the ratio of PCr/ATP.</p><p><b>CONCLUSION</b>In the chronic stage of HF, the expression of UCP2 increases, which causes myocardial energy shortage, SR59230A improves myocardia energy efficiency and cardiac function by means of suppressing the expression of UCP2.</p>